rRNA-mRNA base pairing stimulates a programmed -1 ribosomal frameshift
نویسندگان
چکیده
منابع مشابه
Characterization of the frameshift signal of Edr, a mammalian example of programmed −1 ribosomal frameshifting
The ribosomal frameshifting signal of the mouse embryonal carcinoma differentiation regulated (Edr) gene represents the sole documented example of programmed -1 frameshifting in mammalian cellular genes [Shigemoto,K., Brennan,J., Walls,E,. Watson,C.J., Stott,D., Rigby,P.W. and Reith,A.D. (2001), Nucleic Acids Res., 29, 4079-4088]. Here, we have employed site-directed mutagenesis and RNA structu...
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Title of Document: mRNA SUICIDE: DESTABILIZATION BY PROGRAMMED RIBOSOMAL FRAMESHIFTING Jonathan L. Jacobs, Ph.D., 2006 Directed By: Associate Professor Jonathan D. Dinman, Department of Cell Biology & Molecular Biology Cis-acting mRNA elements that promote programmed -1 ribosomal frameshifting (-1 PRF) redirect a fraction of translating ribosomes into a new translational reading frame. In virus...
متن کاملSaturation mutagenesis of a +1 programmed frameshift-inducing mRNA sequence derived from a yeast retrotransposon.
Errors during the process of translating mRNA information into protein products occur infrequently. Frameshift errors occur less frequently than other types of errors, suggesting that the translational machinery has more robust mechanisms for precluding that kind of error. Despite these mechanisms, mRNA sequences have evolved that increase the frequency up to 10,000-fold. These sequences, terme...
متن کاملIdentification of functional, endogenous programmed −1 ribosomal frameshift signals in the genome of Saccharomyces cerevisiae
In viruses, programmed -1 ribosomal frameshifting (-1 PRF) signals direct the translation of alternative proteins from a single mRNA. Given that many basic regulatory mechanisms were first discovered in viral systems, the current study endeavored to: (i) identify -1 PRF signals in genomic databases, (ii) apply the protocol to the yeast genome and (iii) test selected candidates at the bench. Com...
متن کاملKnotInFrame: prediction of −1 ribosomal frameshift events
Programmed -1 ribosomal frameshift (-1 PRF) allows for alternative reading frames within one mRNA. First found in several viruses, it is now believed to exist in all kingdoms of life. Strong stimulators for -1 PRF are a heptameric slippery site and an RNA pseudoknot. Here, we present a new algorithm KnotInFrame, for the automatic detection of -1 PRF signals from genomic sequences. It finds the ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1994
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.176.22.6842-6851.1994